黄磊，男，博士，副教授，博士生导师，浙江大学求是青年学者。

浙江大学，化学工程与生物工程学院, 生物工程研究所。

电话/传真: 0571-87951220

E-mail: lhuangblue@zju.edu.cn

教育经历

2006年9月，浙江大学生物化工工学博士；2000年6月，浙江大学化学工程学士学位。

工作经历

12/2011 –现在     浙江大学化学工程与生物工程学系生物工程研究所副教授

06/2009 – 12/2011  浙江大学化学工程与生物工程学系生物工程研究所讲师

02/2007 – 05/2009  新加坡南洋理工大学化学与生物分子工程系博士后

主要研究工作

主要的研究方向：1）微生物转录调控和基因组重排新技术；2）重要临床和工业用酶的分子改造；3）合成生物学指导下的基因组进化及其生物产品工程。主持863项目子课题2项、国家自然科学基金青年基金1项、浙江省自然科学基金1项、教育部博士点基金新教师类1项、留学人员科技活动项目择优资助1项。

主要论文：

[1].    L Huang，Y Pu，XL Yang，XC Zhu，J Cai，ZN Xu*，(2015) Engineering of global regulator cAMP receptor protein (CRP) in Escherichia coli for improved lycopene production，J Biotechnol，199（10）：55-61.

[2].    ZM Shi, L Huang*, XT Wu, LP Luo, KJ Xiao, J Cai, ZN Xu*, (2014) Long-term production of butyric acid through immobilization of Clostridium tyrobutyricum in a moving fibrous-bed bioreactor (MFBB),  J. Chem. Technol. Biotechnol.,89(12): 1883-1889

[3].    X Zhang, JY Sheng, L Huang*, LP Du, J Cai, PL Cen, ZN Xu*. (2014) High-level soluble expression of one model olfactory receptor (ODR-10) in Escherichia coli cell-free system. World J. Microbiol. Biotechnol., 30(3): 893-901.

[4].    JY Sheng, L Huang*, XC Zhu, J Cai, ZN Xu*. (2014) Reconstitution of the peptidoglycan cytoplasmic precursor biosynthetic pathway in cell-free system and rapid screening of antisense oligonucleotides for Murenzymes. Appl. Microbiol. Biotechnol., 98(4): 1785-1794.

[5].    X Zhang, JZ Lian, L Kai, L Huang*, PL Cen, ZN Xu*. (2014) Enhanced functional expression of aquaporin Z via fusion of in situ cleavable leader peptides in Escherichia coli cell-free system. Enzyme Microb. Technol., 55: 26-30.

[6].    HQ Chong#, L Huang#, JW Yeow, Ivy Wang, HF Zhang, H Song, RR Jiang*. (2013) Improving Ethanol Tolerance of Escherichia coli by Rewiring Its Global Regulator cAMP Receptor Protein (CRP), PLoS One, 8(2):e57628.

[7].    F Zhu, J Cai, XT Wu, J Huang, L Huang*, JZ Zhu, Q Zheng, PL Cen, ZN Xu*. (2013) The main byproducts and metabolic flux profiling of gamma-PGA-producing strain B. subtilis ZJU-7 under different pH values.  J Biotechnol. 164(1):67-74.

[8].    L Huang, YJ Xiang, J Cai, L Jiang, ZB Lv, YZ Zhang, ZN Xu*. (2011) Effects of three main sugars in cane molasses on the production of butyric acid with Clostridium tyrobutyricum. Korean J. Chem. Eng.. 28(12): 2312-2315

[9].    CL Shu, J Cai, L Huang*, XC Zhu, ZN Xu*.(2011) Biocatalytic production of ethyl butyrate from butyric acid with immobilized Candida rugosa lipase on cotton cloth. J. Mol. Catal. B-Enzym. 72(3-4): 139-144

[10].LQ Zhu, H Chen, L Huang*, J Cai, ZN Xu. (2011) Electrochemical analysis of Clostridium propionicum and its acrylic acid production in microbial fuel cells. Eng. Life Sci.. 11(3):238-244  

[11].W Luo, YP Liu, XC Zhu, WJ Zhao, L Huang*, J Cai, ZN Xu*, PL Cen.(2011)Cloning and characterization of purine nucleoside phosphorylase in Escherichia coli and subsequent ribavirin biosynthesis using immobilized recombinant cells. Enzyme Microb. Technol. 48(6-7): 438-444. 

[12].W Luo, H Chen, LM Fan, L Huang*, ZN Xu, PL Cen. (2011) Adsorption behavior of L-tryptophan on ion exchange resin. Korean J. Chem. Eng.. 28(5): 1272-1279

[13].W Luo, PL Wei, H Chen, LM Fan, L Huang*, ZN Xu, PL Cen. (2011) Kinetics and optimization of L-tryptophan separation with ion-exachange chromatography. Korean J. Chem. Eng.. 28(5): 1280-1285.    

[14].J Huang, YM Du, GH Xu, HL Zhang, F Zhu, L Huang*, ZN Xu. (2011)High yield and cost-effective production of Poly(γ-glutamic acid) with Bacillus subtilis. Eng. Life Sci.. 11(3): 291-297  

[15].DP Zhang, PL Wei, LM Fan, JZ Lian, L Huang*, J Cai, ZN Xu*. (2010) High-level soluble expression of hIGF-1 fusion protein in recombinant Escherichia coli. Process Biochem., 45(8): 1401-1405.

[16].L Huang, PL Wei, LM Fan, D Ye, XC Zhu, ZN Xu*. (2010) The biosynthesis and bioactivity evaluation of the cytosine-substituted mildiomycin analogue (MIL-C) for controlling powder mildew. World J. Microbiol. Biotechnol., 26(4): 649-655.

[17].MH Wang, J Cai, L Huang*, ZB Lv, YZ Zhang, ZN Xu*. (2010) High-level expression and efficient purification of bioactive swollenin in Aspergillus oryzae. Appl. Biochem. Biotechnol , 162 (7): 2027-2036.

[18].L Huang, PL Wei, R Zang, ZN Xu*, PL Cen. (2010) High-throughput screening of high-yield colonies of Rhizopus oryzae for enhanced production of fumaric acid. Ann. Microbiol., 60:287-292.

[19].             L Huang, Leong S.S.J, Jiang R.R. (2009)  Soluble fusion expression and characterization of bioactive human beta-defensin 26 and 27 Appl Microbiol. Biotechnol.84 (2):301-308

[20].             L Huang, Ching C.B., Jiang R.R., Leong S.S.J. (2008)  Production of bioactive human beta-defensin 5 and 6 in Escherichia coli by soluble fusion expression Protein Expr. Purif.61 (2):168-174

[21].             L Huang, Xu Z.N., Zhong Z.X., Peng L., Chen H.Q., Cen P.L. (2007) Enhanced expression and primary purification of soluble HBD3 fusion protein in Escherichia coli Appl Biochem Biotechnol.142 (2):139-147

[22].             L Huang, Ruan H., Gu W.Y., Xu Z.N., Cen P.L. (2007) Functional expression and purification of bovine enterokinase light chain in recombinant Escherichia coliPrep. Biochem. Biotechnol. 37 (3):205-217

[23].L Huang, Wang J.F., Zhong Z.X., Peng L., Chen H.Q. Xu Z.N., Cen P.L. (2006) Production of bioactive human beta-defensin-3 in Escherichia coli by soluble fusion expression Biotechnol. Letters 28:627-632