个人简介

连佳长博士，化学工程与生物工程学院研究员，博士生导师，入选浙江大学“百人计划”。长期从事利用合成生物学的方法构建高效细胞工厂研究工作，i.e.构建了能够富集丙酮酸和乙酰辅酶A的平台细胞工厂；并发展了新的合成生物学工具，用于异源合成途径（i.e. DNA水平-质粒拷贝数；RNA水平-启动子工程；蛋白质水平-RBS工程等）以及宿主细胞代谢调控网络（i.e. 多功能CRISPR/Cas系统）进行快速高效的优化和改造。此外，还从事蛋白质定向进化和无细胞蛋白质合成体系相关工作。共发表论文27篇，其中第一作者/通讯作者文章17篇，包括Nature Communications, Metabolic Engineering, ACS Synthetic Biology, Biotechnology and Bioengineering, Applied Microbiology and Biotechnology等。受邀担任BMC Biotechnology副主编(Associate Editor)以及Metabolic Engineering, Applied Microbiology and Biotechnology, Biotechnology for Biofuels, Microbial Cell Factories, Journal of Industrial Microbiology and Biotechnology, Enzyme and Microbial Technology, Biochemical Engineering Journal, BMC Biotechnology等期刊审稿人。

教育背景

2003-2007，浙江大学，生物工程，学士

2007-2010，浙江大学，生物化工，硕士

2010-2015，伊利诺伊大学香槟分校，化学工程，博士

工作经历

2015-2017，伊利诺伊大学香槟分校，博士后

2017-至今，浙江大学，化学工程与生物工程学院，“百人计划”研究员，博导

研究方向

合成生物学、代谢工程

发表论文

1. Wang H, Liu W, Shi F, Huang L, Lian J*, Qu L, Cai J, Xu Z*. Metabolic Pathway Engineering for High-level Production of 5-Hydroxytryptophan in Escherichia coli. Metab Eng, https://doi.org/10.1016/j.ymben.2018.06.007

2. Lian J, Mishra S, Zhao H*. Recent Advances in Metabolic Engineering of Saccharomyces cerevisiae: New Tools and their Applications. Metab Eng, https://doi.org/10.1016/j.ymben.2018.04.011

3. Schultz C, Lian J, Zhao H. Metabolic Engineering of Saccharomyces cerevisiae Using a Trifunctional CRISPR/Cas System for Simultaneous Gene Activation, Interference, and Deletion. Methods Enzymol, https://doi.org/10.1016/bs.mie.2018.04.010

4. Jiang L, Zhao J, Lian J*, Xu Z* (2018) Cell-Free Protein Synthesis Enabled Rapid Prototyping for Metabolic Engineering and Synthetic Biology. Synth Syst Biotechnol, 3: 90-96

5. Lian J, HamediRad M, Zhao H* (2018) Advancing Metabolic Engineering of Saccharomyces cerevisiae using the CRISPR/Cas System. Biotechnol J, https://doi.org/10.1002/biot.201700601

6. HamediRad M, Lian J, Li H, Zhao H* (2018) RNAi Assisted Genome Evolution Unveils Yeast Mutants with Superior Xylose Utilization. Biotechnol Bioeng, 115: 1552-1560

7. Lian J, Bao Z, Hu S, Zhao H*. Engineered CRISPR/Cas9 System for Multiplex Genome Engineering of Polyploid Industrial Yeast Strains. Biotechnol Bioeng, 115: 1630-1635

8. Lian J, HamediRad M, Hu S, Zhao H* (2017) Combinatorial Metabolic Engineering using an Orthogonal Tri-functional CRISPR System. Nat Commun 8 (1), 1688
   
   

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9. Si T^#, Lian J^#, Zhao H* (2017) Strain Development by Whole-Cell Directed Evolution. Directed Enzyme Evolution: Advances and Applications (Alcalde M, Ed) pp:173–200, Springer International Publishing  (^#Equal Contribution)    

10. Lian J, Jin R, Zhao H* (2016) Construction of Plasmids with Tunable Copy Numbers in Saccharomyces cerevisiae and their Applications in Pathway Optimization and Multiplex Genome Integration. Biotechnol Bioeng 113: 2462–2473

11. Lian J, Zhao H* (2016) Functional Reconstitution of a Pyruvate Dehydrogenase in the Cytosol of Saccharomyces cerevisiae through Lipoylation Machinery Engineering. ACS Synt Biol 5: 689–697

12. Coursolle D, Lian J, Zhao H* (2015) Production of Long Chain Alcohols and Alkanes Upon Coexpression of an Acyl-ACP Reductase and Aldehyde-Deformylating Oxygenase with a Bacterial Type-I Fatty Acid Synthase in E. coli. Mol BioSyst 11: 2464-2472

13. Lian J, Zhao H* (2015) Recent Advances in Biosynthesis of Fatty Acids Derived Products in Saccharomyces cerevisiae via Enhanced Supply of Precursor Metabolites. J Ind Microbiol Biotechnol 42: 437-451

14. Lian J, Zhao H* (2015) Reversal of the β-Oxidation Cycle in Saccharomyces cerevisiae for Production of Fuels and Chemicals. ACS Synt Biol 4: 332-341

15. Feng X, Lian J, Zhao H* (2015) Metabolic Engineering of Saccharomyces cerevisiae to Improve 1-Hexadecanol Production. Metab Eng 27: 10-19

16. Lian J, Si T, Nair NU, Zhao H* (2014) Design and Construction of Acetyl-CoA Overproducing Saccharomyces cerevisiae Strains. Metab Eng 24: 139-149

17. Lian J, Li Y, Hamedi Rad H, Zhao H* (2014) Directed Evolution of a Cellodextrin Transporter for Improved Biofuel Production under Anaerobic Conditions in Saccharomyces cerevisiae. Biotechnol Bioeng 111: 1521-1531

18. Lian J, Chao R, Zhao H* (2014) Metabolic Engineering of a Saccharomyces cerevisiae Strain Capable of Simultaneously Utilizing Glucose and Galactose to Produce Enantiopure (2R,3R)-Butanediol. Metab Eng 23: 92-99

19. Zhang X, Lian J, Kai L, Huang L, Cen P, Xu Z* (2014) Enhanced Functional Expression of Aquaporin Z via Fusion of in situ Cleavable Leader Peptides in Escherichia coli Cell-free System. Enyme Microb Technol 55: 26-30

20. Eriksen D^#, Lian J^#, Zhao H* (2014) Protein Design for Pathway Engineering. J Struct Biol 185(2): 234-242 (^#Equal Contribution) 

21. Du J, Yuan Y, Si T, Lian J, Zhao H* (2012) Customized Optimization of Metabolic Pathways by Combinatorial Transcriptional Engineering. Nucleic Acids Res 40(18): e142

22. Kai L, Kaldenhoff R, Lian J, Zhu X, Dotsch V, Bernhard F, Cen P, Xu Z* (2010) Preparative Scale Production of Functional Mouse Aquaporin 4 Using Different Cell-free Expression Modes. PLoS ONE 5(9): e12972

23. Zhou J, Huang L, Lian J, Sheng J, Cai J, Xu Z* (2010) Reconstruction of the UDP-N-acetylglucosamine biosynthetic pathway in cell-free system. Biotechnol Lett 32:1481-1486

24. Zhang D, Wei P, Fan L, Lian J, Huang L, Cai J, Xu Z* (2010) High-level Soluble Expression of hIGF-1 Fusion Protein in Recombinant Escherichia coli. Process Biochem 45(8): 1401-1405

25. Xu Z*, Lian J, Cai J (2010) Efficient Expression of Aquaporin Z in Escherichia coli Cell-free System Using Different Fusion Vectors. Protein Pept Lett 17(2): 181-185

26. Lian J, Ma Y, Cai J, Wu M, Wang J, Wang X, Xu Z* (2009) High-level Expression of Soluble Subunit b of F₁F₀-ATP Synthase in Escherichia coli Cell-free System. Appl Microbiol Biotechnol 85(2): 303-311

27. Lian J, Ding S, Cai J, Zhang D, Xu Z*, Wang X (2009) Improving Aquaporin Z Expression in Escherichia coli by Fusion Partners and Subsequent Condition Optimization. Appl Microbiol Biotechnol 82(3): 463-470

28. Lian J, Fang X, Cai J, Chen Q, Zheng Q, Kai L, Xu Z* (2008) Efficient Expression of Membrane-Bound Water Channel Protein (Aquaporin Z) in Escherichia coli. Protein Pept Lett 15(7): 687-691